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cDNA cloning of a mouse pituitary adenylate cyclase-activating polypeptide receptor
H Hashimoto1, K Yamamoto, N Hagigara
1Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Biochimica Et Biophysica Acta
|June 11, 1996
Abstract:
A cDNA clone for mouse pituitary adenylate cyclase-activating polypeptide (PACAP) receptor (PACAP-R) was obtained from the brain using reverse transcription-polymerase chain reaction (RT-PCR). The recombinant PACAP receptor expressed in COS cells bound PACAP with about 1000-times higher affinity than vasoactive intestinal polypeptide (VIP), and PACAP stimulated adenylate cyclase through the cloned PACAP receptor. The mouse PACAP receptor consists of 496 amino acids, contains seven transmembrane segments and has 98.4%, 93.0%, and 92.5% identity with the rat, bovine, and human PACAP-R, respectively.