Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An integrated microsatellite length analysis using an automated fluorescent DNA sequencer

Y Toh1, E Oki, S Oda

  • 1Cancer Center, Kyushu University Hospital, Kyushu University, Maidashi, Higashi-ku, Fukuoka, Japan.

Cancer Research
|June 15, 1996
PubMed
Summary

This study introduces a new, automated method for detecting microsatellite instability (MI) in tumors using fluorescent DNA sequencing. This accurate and efficient technique aids in identifying cancer patients at higher risk for second malignant tumors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

T-cell invigoration to cell-free DNA ratio associated with anti-PD-1 response in gastric or gastro-esophageal junction adenocarcinoma: VOYAGER trial.

ESMO gastrointestinal oncology·2026
Same author

Phase III trial of short-course radiotherapy followed by CAPOXIRI versus CAPOX in locally advanced rectal cancer: the ENSEMBLE trial.

ESMO gastrointestinal oncology·2026
Same author

ARCAD-Asia initiative: leveraging yesterday's data for tomorrow.

ESMO gastrointestinal oncology·2026
Same author

Recurrence monitoring using ctDNA in patients with metastatic colorectal cancer: COSMOS-CRC-03 and AURORA studies.

ESMO gastrointestinal oncology·2026
Same author

VOLTAGE-2: multicenter phase II study of nivolumab monotherapy in patients with mismatch repair-deficient resectable locally advanced rectal cancer.

ESMO gastrointestinal oncology·2026
Same author

E7386 in patients with advanced solid tumors: results from the dose-escalation part and an expansion part of a phase I study.

ESMO open·2025

Area of Science:

  • Oncology
  • Genetics
  • Biotechnology

Background:

  • Microsatellite instability (MI) analysis is crucial for identifying cancer patients at risk of developing secondary tumors.
  • Current methods for MI detection can be time-consuming and less accurate.

Purpose of the Study:

  • To develop and validate a novel, automated, and efficient method for detecting microsatellite instability (MI).
  • To assess the feasibility of this new method for routine clinical application in cancer patient follow-up.

Main Methods:

  • Utilized an automated fluorescent DNA sequencer and computer analysis for precise measurement of fluorescent products.
  • Employed dual-fluorescently labeled primers (ROX for tumor, FAM for normal tissue) for simultaneous analysis of microsatellite loci.
  • Detected MI by analyzing distinct fluorescent product size, height, and area patterns.

Related Experiment Videos

Main Results:

  • The new method accurately detected MI in 5 out of 22 (22.7%) gastric cancer cases, consistent with previous radioisotopic findings.
  • Demonstrated the ability to analyze multiple microsatellite loci simultaneously in a single lane.
  • The automated analysis provided rapid and accurate assessment of MI.

Conclusions:

  • The developed automated fluorescent DNA sequencing method offers a more accurate and efficient approach to MI detection.
  • This improved technique holds potential for routine MI screening in cancer patients, aiding in the identification of those at higher risk for second malignant tumors.
  • The method shows promise for clinical application in cancer patient follow-up evaluations.