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The cDNA sequence and infectious transcripts of peanut stripe virus
S Flasinski1, U B Gunasinghe, R A Gonzales
1Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73402, USA.
Gene
|June 1, 1996
Summary
Researchers created a full-length peanut stripe potyvirus (PStV) cDNA clone. Infectious transcripts caused disease and aphid transmissibility, with a coat protein mutation preventing aphid transmission.
Area of Science:
- Plant Virology
- Molecular Biology
- Genetics
Background:
- Peanut stripe potyvirus (PStV) is a significant pathogen affecting peanut crops.
- Understanding PStV's RNA genome and its functional elements is crucial for disease management.
Purpose of the Study:
- To construct and characterize a full-length cDNA clone of the PStV RNA genome.
- To investigate the infectivity and transmissibility of PStV transcripts.
- To elucidate the role of the coat protein in PStV aphid transmission.
Main Methods:
- Construction of a full-length cDNA clone (PStVSF9) of the PStV RNA genome.
- In vitro synthesis of capped PStV transcripts.
- Inoculation of Nicotiana benthamiana plants with transcripts.
- Construction of a coat protein mutant (PStVDAE) and assessment of its properties.
Main Results:
- In vitro-synthesized PStV transcripts were infectious in plants.
- Progeny virus from infectious transcripts was aphid transmissible.
- A PStV mutant with a coat protein mutation (PStVDAE) caused indistinguishable symptoms but lost aphid transmissibility.
Conclusions:
- The full-length PStV cDNA clone is infectious and produces aphid-transmissible virus.
- The PStV coat protein plays a critical role in aphid transmission.
- This study provides a valuable tool for further research into PStV biology and control.