Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mechanical stimulation by intermittent hydrostatic compression promotes bone-specific gene expression in vitro

J Roelofsen1, J Klein-Nulend, E H Burger

  • 1Department of Oral Cell Biology, ACTA-Free University, Amsterdam, The Netherlands.

Journal of Biomechanics
|December 1, 1995
PubMed
Summary

Intermittent hydrostatic compression (IHC) enhances bone formation and specific gene expression in osteoblasts and calvariae cultures. This mechanical stimulation appears crucial for maintaining the differentiated bone cell phenotype.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial neurogastrointestinal encephalomyopathy: Clinical and biochemical impact of allogeneic stem cell transplantation in a Greek patient with one novel <i>TYMP</i> mutation.

Molecular genetics and metabolism reports·2021
Same author

Medical certification of death in South Africa--moving forward.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2015
Same author

Inflammatory factors in the circulation of patients with active rheumatoid arthritis stimulate osteoclastogenesis via endogenous cytokine production by osteoblasts.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2014
Same author

IL-6 alters osteocyte signaling toward osteoblasts but not osteoclasts.

Journal of dental research·2014
Same author

Nitric oxide signaling in mechanical adaptation of bone.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2013
Same author

Growth factor gene expression profiles of bone morphogenetic protein-2-treated human adipose stem cells seeded on calcium phosphate scaffolds in vitro.

Biochimie·2013

Area of Science:

  • Biomechanical Engineering
  • Cell Biology
  • Molecular Biology

Background:

  • Mechanical stimulation is fundamental to bone remodeling, as described by Wolff's Law.
  • Previous studies indicated mechanical stimulation via intermittent hydrostatic compression (IHC) promotes bone formation.
  • The specific effects of IHC on bone-specific gene expression require further elucidation.

Purpose of the Study:

  • To investigate the impact of IHC on alkaline phosphatase (AP) expression and activity.
  • To analyze the modulation of collagen and actin mRNA levels under IHC.
  • To differentiate the cellular responses of osteoprogenitor (OPR) and osteoblast (OB) cells to mechanical stimuli.

Main Methods:

  • Neonatal mouse calvariae and derived OPR and OB cells were cultured.

Related Experiment Videos

  • Intermittent hydrostatic compression (IHC) was applied (0.3 Hz, 13kPa peak stress).
  • AP activity and mRNA levels for AP, collagen, and actin were quantified.

    • Main Results:

    • Control cultures showed decreased AP activity and gene expression over time, except for OPR cell collagen.
    • IHC treatment upregulated AP, collagen, and actin expression and AP activity in calvariae and OB cells.
    • IHC decreased collagen expression in OPR cells, indicating differential cellular responses.

    Conclusions:

    • IHC promotes an osteoblastic phenotype in bone organ and cell cultures.
    • Osteoprogenitor cells exhibit distinct responses to mechanical stress compared to osteoblasts.
    • Mechanical stimuli are vital for maintaining the differentiated phenotype of bone cells, with IHC partially restoring this environment.