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A novel adaptor-related protein complex

F Simpson1, N A Bright, M A West

  • 1Department of Clinical Biochemistry, University of Cambridge, United Kingdom.

The Journal of Cell Biology
|May 1, 1996
PubMed
Summary

Researchers identified a novel coat protein complex, p47, involved in vesicle budding from the trans-Golgi network (TGN). This complex, regulated by ARF, is crucial for protein transport to the plasma membrane via non-clathrin-coated vesicles.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Membrane Trafficking

Background:

  • Coat proteins are essential for vesicle budding in membrane traffic pathways.
  • The components of many coat protein complexes remain unidentified.

Purpose of the Study:

  • To investigate if p47, a homolog of clathrin-coated vesicle adaptin chains, is part of a new coat protein complex.
  • To characterize the function and localization of the p47-containing complex.

Main Methods:

  • Antibodies against p47 were generated.
  • Co-immunoprecipitation was used to identify interacting proteins.
  • Localization studies using immunofluorescence were performed.
  • Regulation by GTPγS and brefeldin A was assessed.

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Main Results:

  • p47 co-immunoprecipitated with beta-NAP and two unknown proteins, forming an adaptor-like heterotetrameric complex.
  • This complex is not enriched in clathrin-coated vesicles.
  • Membrane recruitment of the p47 complex is ARF-dependent and regulated by GTPγS and brefeldin A.
  • The complex localizes to non-clathrin-coated buds and vesicles at the trans-Golgi network (TGN) and plasma membrane in neuronal cells.

Conclusions:

  • The p47-containing complex represents a novel coat for vesicle budding from the TGN.
  • This coat facilitates the transport of newly synthesized proteins to the plasma membrane.