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Adenosine enhances IL-10 secretion by human monocytes
O Le Moine1, P Stordeur, L Schandené
1Department of Gastroenterology, Erasme Hospitol, Free University of Brussels, Belgium.
Journal of Immunology (Baltimore, Md. : 1950)
|June 1, 1996
Summary
Adenosine enhances the anti-inflammatory cytokine Interleukin-10 (IL-10) production in human monocytes. This action contributes to adenosine
Area of Science:
- Immunology
- Cell Biology
- Pharmacology
Background:
- Adenosine is an endogenous anti-inflammatory molecule.
- Its role in regulating cytokine production by monocytes requires further elucidation.
Purpose of the Study:
- To investigate the effect of adenosine on Interleukin-10 (IL-10) production in human monocytes.
- To explore the mechanisms underlying adenosine-mediated cytokine modulation.
Main Methods:
- Human monocytes were stimulated with Tumor Necrosis Factor (TNF), hydrogen peroxide, or Lipopolysaccharide (LPS).
- Adenosine was added at various time points, and IL-10 and TNF levels were measured.
- Adenosine receptor agonists and antagonists were used to investigate receptor involvement.
Main Results:
- Adenosine dose-dependently enhanced IL-10 release from stimulated monocytes.
- Adenosine also inhibited TNF secretion, an effect partially reversed by blocking IL-10.
- These effects were independent of A1 or A2 adenosine receptor activation.
Conclusions:
- Adenosine increases IL-10 secretion by stimulated monocytes via a non-receptor-mediated pathway.
- This mechanism contributes to adenosine's anti-inflammatory properties and may limit tissue injury.