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Porphyromonas gingivalis endotoxin affinity for dental ceramics
F G Robinson1, K L Knoernschild, J D Sterrett
1Department of Oral Rehabilitation, Medical College of Georgia, School of Dentistry, Augusta.
The Journal of Prosthetic Dentistry
|February 1, 1996
Summary
Dental ceramics can bind to Porphyromonas gingivalis lipopolysaccharide (endotoxin), a key factor in periodontal inflammation. Higher endotoxin exposure increases binding and subsequent elution from ceramic surfaces.
Area of Science:
- Biomaterials Science
- Periodontology
- Dental Materials
Background:
- Lipopolysaccharide (endotoxin) from Porphyromonas gingivalis is a potent initiator of biologic responses.
- Dental ceramics are widely used in restorative dentistry, raising questions about their interaction with oral bacteria.
Purpose of the Study:
- To investigate the influence of ceramic composition, surface treatment, and endotoxin dose on lipopolysaccharide adherence and elution.
- To understand the potential role of dental ceramics in sustaining periodontal inflammation.
Main Methods:
- Dental ceramic disks (Opaque, body, Dicor) were exposed to varying concentrations of 3H-lipopolysaccharide (250-2500 EU/ml).
- Adherence was measured after 24-hour incubation, followed by elution assessment in endotoxin-free water for up to 96 hours.
- Surface finish was varied to assess its impact on elution.
Main Results:
- Initial lipopolysaccharide adherence increased with higher exposure doses, ranging from 0.397 to 5.056 EU/mm2.
- Higher initial adherence correlated with greater subsequent elution.
- Ceramic type did not significantly impact elution, but surface finish influenced it at high endotoxin levels.
Conclusions:
- Dental ceramics exhibit an affinity for Porphyromonas gingivalis lipopolysaccharide, potentially contributing to periodontal inflammation.
- Factors like initial endotoxin exposure and surface characteristics influence lipopolysaccharide binding and release from dental materials.