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Expression of FosB during mouse development: normal development of FosB knockout mice
M C Gruda1, J van Amsterdam, C A Rizzo
1Bristol-Meyers Squibb Pharmaceutical Research Institute, Department of Oncology, Princeton, New Jersey 08543-4000, USA.
Oncogene
|May 16, 1996
Summary
FosB protein is widely expressed in developing mouse tissues, particularly bone. While FosB deficiency causes no obvious abnormalities, it impairs transcriptional regulation of certain genes.
Area of Science:
- Molecular Biology
- Developmental Biology
- Genetics
Background:
- FosB is a member of the Fos family, rapidly induced by stimulation, and promotes cell proliferation.
- Understanding FosB's role in mammalian development is crucial.
Purpose of the Study:
- To investigate the tissue distribution of FosB in developing mice.
- To determine the functional role of FosB in mammalian development using knockout models.
Main Methods:
- Immunohistochemistry was used to analyze FosB expression patterns in embryonic and newborn mice.
- FosB-deficient embryonic stem cells, mice, and mouse embryo fibroblasts were generated and analyzed.
Main Results:
- FosB is widely expressed, with highest levels in developing bone (bony and cartilaginous regions), whisker follicles, liver, and epidermis.
- FosB knockout mice show normal fertility and no apparent phenotypic abnormalities.
- FosB-deficient cells exhibit normal proliferation but reduced induction of two AP-1 containing genes upon stimulation.
Conclusions:
- FosB plays a role in transcriptional regulation, specifically in the induction of AP-1 containing genes.
- Despite widespread expression, FosB deficiency does not lead to overt developmental defects in mice, suggesting functional redundancy or context-specific roles.