Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neutralization of Knops system antibodies using soluble complement receptor 1

J M Moulds1, K E Rowe

  • 1Department of Internal Medicine, University of Texas-Houston Medical School, USA.

Transfusion
|June 1, 1996
PubMed
Summary

Recombinant soluble complement receptor 1 (sCR1) effectively neutralizes Knops system antibodies, offering a new method for their identification. This breakthrough aids in precisely identifying Knops system antibodies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

International society of blood transfusion working party on red cell immunogenetics and terminology: report of the Seoul and London meetings.

ISBT science series·2017
Same author

Neurokinin-1 receptor antagonism attenuates neuronal activity triggered by stress-induced reinstatement of alcohol seeking.

Neuropharmacology·2015
Same author

Successful transfusion of antigen positive blood to alloimmunised patients using a monocyte monolayer assay.

Transfusion medicine (Oxford, England)·2015
Same author

International Society of Blood Transfusion Working Party on red cell immunogenetics and blood group terminology: Cancun report (2012).

Vox sanguinis·2013
Same author

International Society of Blood Transfusion Working Party on red cell immunogenetics and blood group terminology: Berlin report.

Vox sanguinis·2011
Same author

The Knops blood-group system: a review.

Immunohematology·2010

Area of Science:

  • Immunology
  • Blood group serology

Background:

  • Knops system antibodies were previously considered nonneutralizable.
  • Knops system antigens are located on complement receptor 1 (CR1).

Purpose of the Study:

  • To investigate if soluble CR1 (sCR1) can neutralize Knops system antibodies.
  • To develop a method for identifying Knops system antibodies.

Main Methods:

  • Radiolabeled immunoprecipitation was used to test sCR1.
  • 45 different antibodies were tested for neutralization with sCR1.
  • Standard serological techniques were employed post-neutralization.

Main Results:

  • sCR1 expressed Kn(a), McC(a), Sl(a), and Yk(a) antigens.
  • All 22 Knops system antibodies tested were neutralized by sCR1.

Related Experiment Videos

  • Other non-Knops alloantibodies showed no decrease in reactivity.

    • Conclusions:

    • Recombinant sCR1 provides an effective method for neutralizing Knops system antibodies.
    • This neutralization technique is a definitive way to identify Knops system antibodies.