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Related Experiment Videos

Evidence for a non-capacitative Ca2+ entry during [Ca2+] oscillations

T J Shuttleworth1, J L Thompson

  • 1Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, NY 14642, USA.

The Biochemical Journal
|June 15, 1996
PubMed
Summary

Calcium (Ca2+) entry in non-excitable cells is not solely dependent on store depletion. This study shows Ca2+ entry control during oscillations differs from current capacitative models.

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Area of Science:

  • Cell biology
  • Physiology
  • Biochemistry

Background:

  • Current models propose capacitative calcium entry (CCE) is activated by intracellular calcium store depletion.
  • Physiological conditions often involve cyclical store refilling and emptying, leading to calcium oscillations.
  • The control of calcium entry during these oscillations is poorly understood.

Purpose of the Study:

  • To investigate the mechanism controlling calcium entry in non-excitable cells during cyclical store refilling and emptying.
  • To determine if capacitative calcium entry models adequately explain calcium entry during calcium oscillations.

Main Methods:

  • Utilized isolated avian nasal gland cells as a model system.
  • Monitored manganese (Mn2+) quench as an indicator of calcium entry.

Related Experiment Videos

  • Manipulated agonist concentrations and store refilling/emptying cycles.
  • Applied protocols designed to detect capacitative calcium entry.

    • Main Results:

    • Agonist-enhanced Mn2+ quench was independent of store cycling during oscillations.
    • Calcium entry pathway remained active even when stores were full and oscillations were inhibited.
    • No calcium entry was detected using a definitive capacitative entry protocol during oscillations.
    • Calcium entry activation preceded detectable store calcium release at low agonist concentrations.

    Conclusions:

    • The control of calcium entry during oscillations is inconsistent with current capacitative models.
    • A non-capacitative mechanism likely governs calcium entry during physiological calcium oscillations.