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Methodology for Accurate Detection of Mitochondrial DNA Methylation
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Methodology for Accurate Detection of Mitochondrial DNA Methylation

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Detection of DNA fragments encompassing the deletion junction of mitochondrial genome

Y Goto1, I Nishino, S Horai

  • 1Department of Ultrastructural Research, National Institute of Neuroscience, Tokyo, Japan.

Biochemical and Biophysical Research Communications
|May 15, 1996
PubMed

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Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...