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Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Published on: February 8, 2016
Identification and characterization of an ecto-ATPase activity in rat Sertoli cells
E Barbacci1, A Filippini, P De Cesaris
1Institute of Histology and General Embryology, School of Medicine, La Sapienza University of Rome, Italy.
Biochemical and Biophysical Research Communications
|May 15, 1996
Abstract:
A membrane associated extracellular ATPase (ecto) has been identified on rat Sertoli cells. Sertoli cell ecto-ATPase demonstrated a Km for ATP of 114 muM and a V(max) of 1.79 mumol/min/2 x 10(5) cells and was activated by either Mg2+ or Ca2+. This ecto-ATPase hydrolyzes other nucleoside triphosphates, but is inactive with ADP. The effects of some possible inhibitors of ectonucleotidases on the breakdown of extracellular ATP by Sertoli cells were also investigated.
