Related Experiment Video
Updated: Aug 9, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
Activation of Pyk2 by stress signals and coupling with JNK signaling pathway
1Department of Pharmacology, New York University Medical Center, 550 First Avenue, New York, NY 10016, USA.
Abstract:
The c-Jun amino-terminal kinase (JNK) is activated by various heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors, inflammatory cytokines, and stress signals. Yet, upstream mediators that link extracellular signals with the JNK signaling pathway are currently unknown. The tyrosine kinase Pyk2 was activated by tumor necrosis factor alpha, by ultraviolet irradiation, and by changes in osmolarity. Overexpression of Pyk2 led to activation of JNK, and a dominant-negative mutant of Pyk2 interfered with ultraviolet light- or osmotic shock-induced activation of JNK. Pyk2 represents a cell type-specific, stress-sensitive mediator of the JNK signaling pathway.
Related Concept Videos
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Amplifying Signals via Enzymatic Cascade
MAPK Signaling Cascades
The JAK-STAT Signaling Pathway
PI3K/mTOR/AKT Signaling Pathway
cAMP-dependent Protein Kinase Pathways

