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45-kDa GTP-binding protein from rat olfactory epithelium: purification, characterization and localization
V I Novoselov1, I V Peshenko, V A Evdokimov
1Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Abstract:
The rat olfactory epithelium contains a specific water-soluble 45-kDa protein. This protein is recognized by anti-peptide antibodies which react with alpha-subunits of the known G-proteins. The 45-kDa protein has been isolated using DEAE-chromatography and gel-exclusion chromatography. The content of 45-kDa protein is about 2% of the total soluble proteins of the olfactory mucosa and it is located at the mucociliary surface. According to photo-affinity labeling, the 45-kDa protein possesses a high affinity to GTP and exhibits low GTP hydrolytic activity. The functions of the 45-kDa protein are discussed.