Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple p21ras effector pathways regulate nuclear factor of activated T cells

E Genot1, S Cleverley, S Henning

  • 1Lymphocyte Activation Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.

The EMBO Journal
|August 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Classification of Kudoa thyrsites infected and uninfected fish using a handheld near-infrared spectrophotometer, SIMCA and PLS-DA.

Journal of fish diseases·2024
Same author

Immunoglobulin G/immunoglobulin M autoantibody ratios in incomplete systemic lupus erythematosus.

Scandinavian journal of rheumatology·2024
Same author

Evaluation of four disease management programs: evidence from blue cross blue shield of Louisiana.

Journal of medical economics·2020
Same author

Reduced GLP-1R expression in gastric glands of patients with type 2 diabetes mellitus.

The Journal of clinical endocrinology and metabolism·2014
Same author

UVA-induced DNA double-strand breaks result from the repair of clustered oxidative DNA damages.

Nucleic acids research·2012
Same author

The ground state phase diagram of the diluted ferromagnetic Kondo-lattice model.

Journal of physics. Condensed matter : an Institute of Physics journal·2011

p21ras and calcium signals regulate Nuclear Factor of Activated T cells (NFAT) in T cells. This regulation requires both MAP kinase ERK-2 and Rac-1 pathways, highlighting multiple effector pathways for NFAT induction.

Area of Science:

  • Molecular Biology
  • Immunology
  • Cell Signaling

Background:

  • Nuclear Factor of Activated T cells (NFAT) is crucial for IL-2 gene expression.
  • NFAT induction is regulated by p21ras and calcium signaling pathways.

Purpose of the Study:

  • To investigate the role of the MAP kinase ERK-2 pathway in NFAT regulation.
  • To elucidate the involvement of Rac-1 in p21ras-mediated NFAT induction.

Main Methods:

  • Utilized dominant-negative and constitutively active forms of MAPKK-1 and Rac-1.
  • Examined the effects on NFAT induction, ERK-2 activation, and AP-1 complex formation.

Main Results:

  • Dominant-negative MAPKK-1 inhibited NFAT induction.

Related Experiment Videos

  • Constitutively active MAPKK-1 activated ERK-2 and Elk-1 but did not fully induce NFAT.
  • Dominant-negative N17Rac blocked TCR and p21ras activation of NFAT without affecting ERK-2.
  • Activated Rac-1 induced AP-1 but not NFAT.
  • Combined MAPKK-1 and Rac-1 activation could not substitute for p21ras in NFAT induction.

    • Conclusions:

    • p21ras regulation of NFAT in T cells depends on multiple effector pathways.
    • Both MAPKK-1/ERK-2 and Rac-1 pathways are essential for p21ras-mediated NFAT induction.