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Molecular cloning of murine decay accelerating factor by immunoscreening
International Immunology
|March 1, 1996
Summary
Researchers successfully cloned the mouse decay accelerating factor (DAF) cDNA using immunoscreening. This finding enables new studies on the in vivo role of DAF in autoimmune diseases.
Area of Science:
- Immunology
- Molecular Biology
Background:
- The human decay accelerating factor (DAF) cDNA was cloned in 1987.
- Previous attempts to clone mouse DAF cDNA via cross-hybridization were unsuccessful.
Purpose of the Study:
- To clone the cDNA of mouse decay accelerating factor (DAF).
- To enable further research into the in vivo functions of DAF.
Main Methods:
- Immunoscreening using rabbit antiserum against purified mouse DAF.
- DNA sequencing and sequence identity analysis.
Main Results:
- Successfully cloned mouse DAF cDNA containing four short consensus repeats (SCR).
- Mouse DAF cDNA showed 63.7-63.8% base sequence identity and 47.2-46.5% amino acid identity to human and guinea pig DAF.
- Identified significant sequence homology between mouse DAF and Crry/p65.
Conclusions:
- The identification of mouse DAF opens avenues for in vivo studies.
- Future research can utilize autoimmune mice and DAF gene knockout models.