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Related Experiment Videos

CD40 expression and function in murine B cell ontogeny

E Castigli1, F Young, A M Carossino

  • 1Division of Immunology, Children's Hospital, Boston, MA 02115, USA.

International Immunology
|March 1, 1996
PubMed
Summary
This summary is machine-generated.

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CD40 antigen is crucial for B cell activation. Its expression and function emerge during B cell development, with bcl-2 influencing pre-B cell responses to CD40 signaling.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • CD40 antigen is a key receptor in the tumor necrosis factor receptor superfamily.
  • It is expressed on mature B lymphocytes and is vital for B cell activation, isotype switching, and germinal center formation.
  • Understanding CD40's role during B cell development is crucial for immunology research.

Purpose of the Study:

  • To investigate CD40 antigen expression and function during mouse B cell development.
  • To analyze the impact of specific genetic modifications on B cell development and CD40 signaling.
  • To determine the stage-specific roles of CD40 in B cell maturation.

Main Methods:

  • Analysis of CD40 expression and function in normal mice.
  • Utilizing transgenic mouse models with arrested B cell development (RAG-2-/-).

Related Experiment Videos

  • Examining transgenic mice with altered expression of mu heavy chain and/or bcl-2 proto-oncogene.

    • Main Results:

    • CD40 antigen was not detected on pro-B cells but was expressed at low levels on pre-B cells.
    • Pre-B cells did not respond to CD40 triggering (CD23 expression or proliferation with IL-4).
    • Overexpression of bcl-2 increased CD40 density on pre-B cells, enabling response to CD40 ligation.

    Conclusions:

    • CD40 expression and functional responsiveness are acquired during B cell development.
    • The bcl-2 proto-oncogene significantly modulates CD40 expression density and functional responses in pre-B cells.
    • These findings provide insights into the intricate regulation of B cell activation and development.