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Updated: Aug 9, 2026

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Published on: September 28, 2018
Ion channel permeable for divalent and monovalent cations in native spinach thylakoid membranes
1Julius-von-Sachs Institut für Biowissenschaften der Universität Würzburg, Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany.
Abstract:
A cation-selective channel was characterized in isolated patches from osmotically swollen thylakoids of spinach (Spinacea oleracea). This channel was permeable for K+ as well as for Mg2+ and Ca2+ but not for Cl-. When K+ was the main permeant ion (symmetrical 105 mM KCl) the conductance of the channel was about 60 pS. The single channel conductance for different cations followed a sequence K+ > Mg2+ >/= Ca2+. The permeabilities determined by reversal potential measurements were comparable for K+, Ca2+, and Mg2+. The cation channel displayed bursting behavior. The total open probability of the channel increased at more positive membrane potentials. Kinetic analysis demonstrated that voltage dependence of the total open probability was determined by the probability of bursts formation while the probability to find the channel in open state within a burst of activity was hardly voltage-dependent. The cation permeability of intact spinach thylakoids can be explained on the single channel level by the data presented here.
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