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Updated: Aug 15, 2026
qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 9, 2014
[Sequence analysis of PCR amplification products of old formalin fixed conisation tissue sections]
1Universitäts-Frauenklinik Mannheim, Klinikum der Stadt Mannheim, Fakultät für Klinische Medizin, Universität Heidelberg.
Objective:
Standard analytical techniques (DNA hybridisation methods) require high-quality DNA. In very small samples and in processed tissue (e.g. analysis of DNA in paraffin-embedded archival tissues) these methods fail due to their restricted sensitivity.
Methods:
In this study we used the considerably more sensitive PCR for analysis of HPV-16-e6/-e7 DNA in histologic cone biopsy sections. HPV-16-e6 and -e7 ORF were amplified, subcloned into M13mp18 and sequenced.
Results:
The sequence is identical to the published e6/e7 sequence.
Conclusions:
Due to its high sensitivity the PCR is suitable to give answers to questions in cellular microbiology even in formalin-fixed paraffin-embedded tissues.
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