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Updated: Aug 19, 2026
Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates
Published on: May 9, 2016
[Comparison of different methods for determination of proliferative activity of breast carcinomas]
D Mink1, C Villena-Heinsen, I Tossounidis
1Universitätsfrauenklinik und Poliklinik mit Hebammenlehranstalt Homburg/Saar.
Objectives:
Different methods for evaluation of nuclear atypia and proliferative activity were compared in fifty cases of breast cancer.
Methods:
Beneath histopathologic grading, ploidy of the stem cell line and S-phasefraction were measured by flow cytometry. Ki67-index was determined immunohistochemically and by image cytometry on smears grade of malignancy and 2cDI were measured.
Results:
Only Ki67-index was correlated with histopathological grading. The various proliferative indices (Ki67-index, S-phase-fraction, 2cDI) were not correlated. Between image- and flow-cytometric DNA-analysis significant differences were found.
Conclusions:
With the immunohistochemical determination of Ki67-index in conjunction with image-cytometry a morphologic control of the material investigated is possible. So in comparison with flow-cytometry a more differentiated analysis can be performed.
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