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[Spermatozoa antigen SAA-1 and co-precipitating molecules: regulation of human acrosome reaction]
C Brucker1, G v Herder, A Keberle
1I. Frauenklinik, Ludwig-Maximilians-Universität, München.
Objectives:
Examination of the regulation of the human sperm acrosome reaction as an example of ligand-induced exocytosis.
Methods:
Development of monoclonal antibodies (mab) against immunaffinity-purified sperm antigen SAA-1. Examination of cross-reactivity of the mabs to human tissues using immunohistochemistry. Examination of reactivity to endometrial carcinoma cell lines and to PC-12 cells by immunohistochemistry and by radioimmunoassay.
Results:
Mabs to immunoaffinity-purified SAA-1 recognize three coprecipitating molecules. Cross-reactivity was demonstrated to glandular epithelia exhibiting ligand-induced exocytosis, and to endometrial carcinoma and PC-12 cells.
Conclusions:
The proteins described could be components of the exocytosis-regulating machinery of human spermatozoa.