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[Modification of chorionic carcinoma cells by immunomodulators. Cytokines and chorionic carcinoma cells]
C Marth1, P Berger, H Zwierzina
1Univ. Klinik für Frauenheilkunde, Innsbruck.
Objectives:
The role of inflammatory cytokines on regulation of hCG biosynthesis was studied.
Methods:
JAR choriocarcinoma cells were cultured and treated with interleukin-1 (IL-1), IL-3. IL-4, stem cell factor (SCF), tumor necrosis factor (TNF), and granulocyte or granulocyte-macrophage-colony stimulating factor. hCG concentration was determined by immunoradiometric methods and cell number was enumerated by means of an electronic particle counter.
Results:
Il-1 and TNF increased biosynthesis of holo-hCG as well as the free alpha- and free beta-subunit and the beta-core protein. Proliferation was not affected by any cytokine tested.
Conclusion:
Inflammatory cytokines are able to increase the biosynthesis of hCG.