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Reversed-phase liquid chromatography on a microspherical carbon column at high temperature
1Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Japan.
Journal of Chromatography. A
|March 29, 1996
Summary
High-temperature chromatography using spherical carbon columns up to 160°C is stable for durable compounds. Peptide bonds remain stable for analysis, but indole residues degrade under these conditions.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- High-performance liquid chromatography (HPLC) often requires elevated temperatures for improved separation.
- Spherical carbon columns offer unique selectivity but their high-temperature stability is crucial.
Purpose of the Study:
- To describe chromatographic conditions for spherical carbon columns at high temperatures (up to 160°C).
- To evaluate the stability of the column and sample components under these conditions.
Main Methods:
- Utilized a spherical carbon column with a specialized heating and eluate cooling system.
- Investigated the stability of peptide bonds and indole residues at high temperatures and acidity.
Main Results:
- The spherical carbon column demonstrated stability at temperatures up to 160°C.
- Peptide bonds were found to be stable enough for analysis.
- Indole residues exhibited poor stability under the tested high-temperature and acidic conditions.
Conclusions:
- Spherical carbon columns are applicable for analyzing thermally stable compounds at high temperatures.
- Care must be taken when analyzing samples containing acid-labile residues like indole.