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Updated: Jul 10, 2026

Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens
Published on: March 14, 2019
Efficient expression, processing and secretion of a biologically active mammalian protein by Vibrio cholerae
1Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
Abstract:
The use of Vibrio cholerae as a secretory expression system for the expression of a mammalian protein, namely human growth hormone, under the control of the heat labile enterotoxin chain B signal sequence is reported. The protein is efficiently expressed and processed. The mature protein is exported to the periplasm after which it is secreted to the extracellular milieu. The expressed and secreted hGH actively binds to its receptor as established by its receptor binding activity. The biological activity of the protein is demonstrated in vitro in a Nb2 proliferation assay.
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