Establishment of immunological probes to study human gonadotropin-releasing hormone receptors
A A Karande1, K Rajeshwari, D J Schol
1Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Molecular and Cellular Endocrinology
|October 30, 1995
Abstract:
Monoclonal antibodies were raised to a synthetic peptide corresponding to amino acids 1-29 of the human gonadotropin-releasing hormone (GnRH) receptor. One of the two antibodies was found to recognise GnRH receptors on human pituitary gonadotrophs as determined by immunohistochemistry and supported by Western blotting. The antibody also bound to T47D human breast carcinoma cell line as determined by flow cytometric analysis.
