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Updated: Feb 15, 2026

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
Published on: June 7, 2020
Determination of synthetic estrogens in illegal veterinary formulations by HPTLC and HPLC
E S Nascimento1, M C Salvadori, L M Ribeiro-Neto
1Departmento de Análises Clínicas e Toxicology, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo.
Abstract:
To determine the actual amount of diethylstilbestrol, hexestrol, and dienestrol in formulations such as pellets and oily injections that are illegally available on the Brazilian market, a simple methanol extraction is used for the analysis of the pellets and an ether extraction with Sephadex columns (for clean-up) is used for the oily injections. High-performance thin-layer chromatography is used for identification (as a qualitative and semiquantitative method), and high-performance liquid chromatography is used for quantitation. The results of the analysis show that all the formulations are not in accordance with the information listed on their labels.
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