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Related Experiment Videos

Lymphocytic and collagenous colitis: an immunohistochemical study

J F Mosnier1, L Larvol, J Barge

  • 1Service d'Anatomie et de Cytologie Pathologiques, Hôpital Beaujon, Clichy, France.

The American Journal of Gastroenterology
|April 1, 1996
PubMed
Summary

Immune abnormalities in lymphocytic colitis (LC) and collagenous colitis (CC) are similar, involving increased CD 8 intraepithelial lymphocytes (IELs) and abnormal HLA DR expression. These findings suggest a shared MHC-restricted immune mechanism distinct from celiac disease.

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Area of Science:

  • Gastroenterology
  • Immunology
  • Colorectal Pathology

Background:

  • Lymphocytic colitis (LC) and collagenous colitis (CC) share increased intraepithelial lymphocytes (IELs).
  • Similarities suggest a potential common underlying immune mechanism.
  • Investigating IEL and lamina propria lymphocyte phenotypes is crucial.

Purpose of the Study:

  • To determine the phenotype of IELs and lamina propria lymphocytes in LC and CC.
  • To compare immune cell profiles between LC, CC, and controls.
  • To elucidate potential shared pathogenic pathways.

Main Methods:

  • Immunohistochemical analysis of colonic and ileal biopsies from LC, CC, and control groups.
  • Utilized monoclonal antibodies targeting T-cells (CD4, CD8), HLA DR, and T-cell receptor (TcR) variants (alpha beta, gamma delta).

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  • Examined IELs and lamina propria lymphocytes across all bowel segments.

    • Main Results:

    • Both LC and CC showed increased IEL counts, with a predominance of CD 8 IELs over CD 4 IELs.
    • Most IELs expressed TcR alpha beta; TcR gamma delta-bearing cells were not elevated.
    • CD 4+ helper T-cells were predominant in the lamina propria, and epithelial cells abnormally expressed HLA DR antigens.

    Conclusions:

    • Immune abnormalities in LC and CC are similar, pointing to a potential MHC-restricted immune mechanism.
    • Evidence includes CD 4+ T-cell accumulation, CD 8 TcR alpha beta IEL-related epithelial damage, and abnormal HLA DR expression.
    • The immune mechanisms in LC/CC likely differ from those in celiac disease.