Related Experiment Video
Updated: Aug 8, 2026

Studying Protein Import into Chloroplasts Using Protoplasts
Published on: December 10, 2018
Chloroplast biogenesis 72: a [4-vinyl]chlorophyllide a reductase assay using divinyl chlorophyllide a as an exogenous
1Laboratory of Plant Pigment Biochemistry and Photobiology, University of Illinois, Urbana 61801, USA.
Abstract:
[4-Vinyl]Chlorophyllide alpha reductase (4VCR) catalyzes the conversion of 2,4-divinyl chlorophyllide alpha (DV Chlide alpha) to 2-vinyl,4-ethyl chlorophyllide alpha (MV Chlide alpha) via an NAPDH- dependent reaction. MV Childe alpha is the immediate precursor of monovinyl chlorophyll alpha in plants. In etiolated cucumber (Cucumis sativus L.) cotyledons, 4VCR is a plastidic membrane-bound enzyme. Further research on this enzyme required the development of an assay that utilizes DV Childe alpha as an exogenous substrate. Such an assay is now described. It involves conversion of exogenous DV Chlide alpha to MV Chlide alpha at high rates by etioplast membranes of cucumber, corn (Zea mays L.), and barley (Hordum vulgare L.). 4VCR exhibits high activity between 30 and 40C and in the pH range of 6.3 to 7.0. Activity is quasilinear for the first 60 s of incubation.
More Related Videos
10:28Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Published on: October 19, 2018
07:58Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation
Published on: January 12, 2024
Related Concept Videos
Anatomy of Chloroplasts
Photosystem II
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
The Calvin Cycle
The Anatomy of Chloroplasts
Structure of Chloroplasts
A...
The Calvin Benson Cycle
The Z-Scheme of Electron Transport in Photosynthesis