Neurons and glial cells of the enteric nervous system: studies in tissue culture

The enteric nervous system (ENS) has been recognized as the main component in regulating the function of the digestive tract and as a model for studying neuronal physiology and pharmacology. Most of the present knowledge on the ENS was derived from in vitro studies on freshly isolated plexuses. In 1978 the first study on cultured myenteric neurons was published and since then there has been a growing interest in this method. Several different culture preparations have been introduced, including the recent development of cultures from adult guinea-pigs and humans. This review summarizes the findings which have been made using cultured enteric neurons and glia. The main topics that are described are the role of the extracellular matrix and of hormones on neuronal growth, neuron-glia interactions, release of neuropeptides and their actions on neurons and co-transmission between neurons.