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Related Experiment Videos

High-concentration ethanol production from cooked corn starch by using medium-temperature cooking process

Z Chi1, J Liu, P Xu

  • 1Department of Microbiology, Shandong University, Jinan, China.

Chinese Journal of Biotechnology
|January 1, 1995
PubMed
Summary

This study details an enhanced process for producing high-concentration ethanol from corn starch using Saccharomyces sp. W4. Adding ammonium sulfate significantly boosted ethanol yield and fermentation speed.

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Area of Science:

  • Biotechnology
  • Fermentation Science
  • Biochemical Engineering

Background:

  • Traditional ethanol production from corn starch faces challenges in achieving high concentrations.
  • Optimizing enzymatic hydrolysis and yeast fermentation is crucial for efficient biofuel production.

Purpose of the Study:

  • To develop an improved process for high-concentration ethanol production from cooked corn starch.
  • To evaluate the impact of ammonium sulfate supplementation on fermentation efficiency.

Main Methods:

  • Simultaneous cooking and liquefaction of corn starch using thermophilic alpha-amylase (95-105°C).
  • Saccharification of the mash using high-efficiency glucoamylase (60°C).
  • Fermentation by Saccharomyces sp. W4, with and without ammonium sulfate addition.

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Main Results:

  • Saccharomyces sp. W4 achieved 18.3% (v/v) ethanol concentration within 60 hours.
  • Supplementation with 0.9 g ammonium sulfate per 280 mL increased ethanol yield to 18.9% (v/v) within 50 hours.
  • Ammonium sulfate addition reduced residual sugars, indicating more complete fermentation.

Conclusions:

  • The developed process is effective for high-concentration ethanol production from corn starch.
  • Ammonium sulfate is a beneficial additive for accelerating fermentation and improving ethanol yield.
  • This method offers a promising route for efficient bioethanol manufacturing.