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Biosensor based on chemiluminescence for serum uric acid determination

J Liu1, J Guo, G Li

  • 1Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Chinese Journal of Biotechnology
|January 1, 1995
PubMed
Summary

A novel biosensor accurately measures serum uric acid using a luminol chemiluminescent reaction. This efficient method offers rapid results and long-term stability for clinical diagnostics.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Elevated serum uric acid levels are associated with various health conditions, including gout and kidney disease.
  • Accurate and rapid determination of serum uric acid is crucial for effective patient management.
  • Existing methods may have limitations in terms of speed, cost, or complexity.

Purpose of the Study:

  • To develop and evaluate a novel biosensor for the quantitative determination of serum uric acid.
  • To assess the performance characteristics of the developed biosensor, including response time, linear range, and precision.
  • To compare the biosensor's performance with established analytical methods.

Main Methods:

  • Development of a biosensor utilizing a luminol chemiluminescent reaction.
  • Immobilization of uricase onto a column for sample processing.
  • Testing with various concentrations of serum uric acid and comparison to a standard colorimetric method.

Main Results:

  • The biosensor demonstrated a rapid response time of 47 seconds with a 17-microliter sample volume, enabling 40 samples per hour.
  • A wide linear range from 1 mg/dL to 20 mg/dL was established, with good within-day (3.22-4.36%) and day-to-day (6.18-7.80%) imprecision.
  • High recovery rates (93-109%) and excellent correlation (r=0.9909) with the standard method were observed.
  • The immobilized uricase column maintained 94% of its activity after over 2000 runs, indicating significant operational stability.

Conclusions:

  • The developed luminol chemiluminescent biosensor provides a sensitive, rapid, and stable method for serum uric acid determination.
  • This biosensor shows excellent performance characteristics, comparable or superior to existing methods.
  • The long-term stability of the immobilized enzyme column suggests its potential for routine clinical use.

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