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One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin
1QIAGEN Inc., Chatsworth, CA 91311, USA.
Molecular Biotechnology
|December 1, 1995
Abstract:
The 6xHis/Ni-NTA system allows rapid and efficient affinity purification of recombinant proteins from virtually any expression system. Protocols and tips for purification under both native and denaturing conditions are provided, as well as a rapid spin procedure for protein minipreps.