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A general assay for restriction endonucleases and other DNA-modifying enzymes with plasmid substrates
I B Vipond1, G S Baldwin, M Oram
1Department of Biochemistry, Centre for Molecular Recognition, University of Bristol, UK.
Molecular Biotechnology
|December 1, 1995
Abstract:
A procedure for measuring the activities of enzymes that alter the covalent structure of DNA is described. The assay utilizes covalently closed circles of DNA as the substrate and yields quantitative data on the fraction of this DNA converted to both open-circle and linear forms.