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Acinetobacter baumannii outbreak isolates characterized by three typing methods
1Service de Bacteriologie, CHRU Hôpital Bellevue, Saint-Etienne, France.
Abstract:
Forty-two strains of Acinetobacter baumannii were isolated from 15 patients hospitalized in a French intensive care unit. An epidemiological study based on the typing of these isolates was carried out using biotyping, antibiotyping, and ribotyping to recognize the transmission of multiresistant strains by transfer of a patient from one hospital to another. Fifteen strains from the outbreak (1 strain for each patient), five Acinetobacter baumannii strains isolated before the outbreak in Bellevue Hospital (St. Etienne), and five strains isolated in Cochin Hospital (Paris) were included. The three methods gave a good correlation: the epidemic strains had the same antibiotic resistance pattern, the same biotype, and the same ribotypes obtained with three different endonucleases.
Insights
Multiresistant Acinetobacter baumannii strains were tracked in a French intensive care unit using biotyping, antibiotyping, and ribotyping. These methods confirmed the transmission of specific epidemic strains between hospitals.
Area of Science:
- Microbiology
- Infectious Diseases
- Epidemiology
Background:
- Acinetobacter baumannii is a significant opportunistic pathogen, frequently causing hospital-acquired infections, particularly in intensive care units.
- Multiresistant strains of Acinetobacter baumannii pose a substantial threat to patient health and complicate treatment strategies.
Purpose of the Study:
- To investigate the transmission dynamics of multiresistant Acinetobacter baumannii strains within a French intensive care unit.
- To evaluate the effectiveness of combined typing methods for epidemiological surveillance of Acinetobacter baumannii.
Main Methods:
- Biotyping, antibiotyping, and ribotyping were employed to characterize Acinetobacter baumannii isolates.
- Isolates were collected from patients during an outbreak, as well as from historical collections from different hospitals.
Main Results:
- The study successfully identified specific epidemic strains of Acinetobacter baumannii.
- Biotyping, antibiotyping, and ribotyping demonstrated high concordance in identifying and correlating the epidemic strains.
- Consistent antibiotic resistance patterns, biotypes, and ribotypes were observed among the outbreak strains.
Conclusions:
- Combined microbiological typing methods are effective for tracing the transmission of multiresistant Acinetobacter baumannii.
- Patient transfer between healthcare facilities can facilitate the spread of epidemic Acinetobacter baumannii strains.