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HLA-D typing with homozygous lymphoblastoid cell lines
Zeitschrift Fur Immunitatsforschung. Immunobiology
|April 1, 1977
Summary
Established lymphoblastoid cell lines (LCL) from homozygous siblings are effective for human leukocyte antigen (HLA)-D typing. These LCLs accurately identify HLA-D determinants present on peripheral blood lymphocytes (PBL).
Area of Science:
- Immunogenetics
- Cell Biology
Background:
- Human Leukocyte Antigen (HLA) typing is crucial for transplantation and disease association studies.
- Peripheral Blood Lymphocytes (PBL) are traditionally used for HLA-D typing.
- Establishing reliable and accessible cell lines for HLA typing is an ongoing area of research.
Purpose of the Study:
- To investigate the utility of lymphoblastoid cell lines (LCL) derived from homozygous donors for HLA-D typing.
- To compare the efficacy of LCLs versus PBLs in HLA-D determinant identification.
Main Methods:
- Established lymphoblastoid cell lines (LCL) from two HLA-DW3 homozygous siblings.
- Performed HLA-D typing using both homozygous LCL and PBL.
- Utilized a cohort of 51 unrelated individuals selected based on HLA-A,B locus antigens.
Main Results:
- All individuals positive for DW3 using PBL typing cells were also positive using LCL typing cells.
- The two homozygous LCLs demonstrated highly similar performance across the tested population.
- HLA-D determinants were consistently represented on both LCL and PBL cells.
Conclusions:
- Lymphoblastoid cell lines derived from homozygous donors are suitable for HLA-D typing.
- LCLs offer a viable alternative to PBLs for HLA-D typing, ensuring consistent representation of determinants.