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Related Experiment Videos

Soft-X-ray damage to biological samples

H Fujisaki1, S Takahashi, H Ohzeki

  • 1Nikon Corporation, Tsukuba Research Laboratory, Ibaraki, Japan. fujisaki@tsukubagw.nikon.co.jp

Journal of Microscopy
|May 1, 1996
PubMed
Summary

Investigating X-ray damage in the water-window (2.4-4.3 nm), this study found that biological samples like yeast cells and myofibrils are damaged at lower X-ray fluxes than previously thought for high-resolution microscopy.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • X-ray microscopy offers high resolution for biological samples.
  • Understanding X-ray damage is crucial for sample preservation.
  • The 'water-window' (2.4-4.3 nm) is a key region for X-ray microscopy.

Purpose of the Study:

  • To investigate X-ray damage to biological samples within the 2.7-5 nm wavelength range.
  • To determine the X-ray flux thresholds causing damage to yeast cells and myofibrils.
  • To compare damage thresholds with requirements for high-resolution X-ray microscopy.

Main Methods:

  • Exposure of yeast cells and myofibrils to X-rays from a laser-plasma source.
  • Utilizing a Wolter mirror condenser and sample cell for X-ray delivery.

Related Experiment Videos

  • Assessing biological damage via dye exclusion (yeast) and contractility (myofibrils).

    • Main Results:

    • Yeast cells lost dye exclusion ability above 1 x 10(6) photons/micron-2.
    • Myofibrils lost contractility above 4 x 10(5) photons/micron-2.
    • These damage thresholds are lower than those needed for high-resolution X-ray microscopy.

    Conclusions:

    • X-ray damage occurs at lower fluxes than anticipated for high-resolution imaging.
    • Careful consideration of X-ray flux is necessary to preserve sample integrity.
    • Findings impact protocols for X-ray microscopy of biological specimens.