Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence variation as a strategy for crystallizing RNA motifs

A C Anderson1, B E Earp, C A Frederick

  • 1Committee on Higher Degrees in Biophysics, Harvard University and Dana-Farber Cancer Institute, Boston, MA 02115, USA.

Journal of Molecular Biology
|June 21, 1996
PubMed
Summary

Obtaining high-quality RNA crystals for X-ray crystallography is challenging. This study details methods to improve crystal size and diffraction, highlighting sequence variation as key for better crystal quality in structural biology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence for the Collective Nature of Radial Flow in Pb+Pb Collisions with the ATLAS Detector.

Physical review letters·2026
Same author

Evidence for the Dimuon Decay of the Higgs Boson in pp Collisions with the ATLAS Detector.

Physical review letters·2025
Same author

Evidence for Longitudinally Polarized W Bosons in the Electroweak Production of Same-Sign W Boson Pairs in Association with Two Jets in pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

Physical review letters·2025
Same author

Observation of tt[over ¯] Production in Pb+Pb Collisions at sqrt[s_{NN}]=5.02  TeV with the ATLAS Detector.

Physical review letters·2025
Same author

Search for Dark Matter Produced in Association with a Dark Higgs Boson in the bb[over ¯] Final State Using pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

Physical review letters·2025
Same author

Search for Magnetic Monopole Pair Production in Ultraperipheral Pb+Pb Collisions at sqrt[s_{NN}]=5.36  TeV with the ATLAS Detector at the LHC.

Physical review letters·2025

Area of Science:

  • Structural biology
  • Biochemistry
  • Crystallography

Background:

  • X-ray crystallography is a powerful method for determining RNA structures.
  • Current limitations in RNA crystallography stem from difficulties in obtaining large, high-quality crystals.

Purpose of the Study:

  • To outline techniques for improving the success rate of RNA crystallization.
  • To enhance the size and diffraction limits of RNA crystals.
  • To identify factors that influence RNA crystal quality.

Main Methods:

  • Review of established and novel techniques for RNA crystal growth.
  • Exploration of strategies to optimize crystallization conditions.
  • Analysis of the impact of sequence variation on crystal properties.

Related Experiment Videos

Main Results:

  • Sequence variations are more effective than crystallization condition variations in improving crystal quality.
  • Specific techniques can enhance the likelihood of obtaining suitable RNA crystals.
  • Methods exist to extend the diffraction limits of RNA crystals.

Conclusions:

  • Improving RNA crystal quality is crucial for advancing RNA structure determination via X-ray crystallography.
  • Strategic sequence modification offers a promising avenue for overcoming crystallization challenges.
  • Further development of crystallization techniques will enhance the structural characterization of RNA molecules.