Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Cold preservation of human sperm]

W D Junge, K H Rätz, E Clasen

    Zentralblatt Fur Gynakologie
    |January 1, 1977
    PubMed
    Summary

    A novel technique for human sperm freezing and storage was developed, offering low technical cost. This method achieved 50% motility recovery in samples, showing promise for assisted reproduction.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Magma Chamber Response to Ice Unloading: Applications to Volcanism in the West Antarctic Rift System.

    Geochemistry, geophysics, geosystems : G(3)·2024
    Same author

    Hemispherical differences in the shape and topography of asteroid (101955) Bennu.

    Science advances·2020
    Same author

    Acquisition of rock phosphate by combined application of ammonium fertilizers and Bacillus amyloliquefaciens FZB42 in maize as affected by soil pH.

    Journal of applied microbiology·2020
    Same author

    Risk assessment of recent Egyptian H5N1 influenza viruses.

    Scientific reports·2016
    Same author

    [Repetitive transcranial magnetic stimulation for the upper limb motor function improvement after stroke].

    Fortschritte der Neurologie-Psychiatrie·2014
    Same author

    Root exudation and root development of lettuce (Lactuca sativa L. cv. Tizian) as affected by different soils.

    Frontiers in microbiology·2014

    Area of Science:

    • Reproductive biology
    • Cryobiology
    • Andrology

    Context:

    • Spermatozoa cryopreservation is crucial for assisted reproductive technologies.
    • Existing methods face challenges in efficiency and cost-effectiveness.
    • Standardized protocols for human sperm freezing are continuously being refined.

    Purpose:

    • To develop and evaluate a new, cost-effective technique for human spermatozoa freezing and storage.
    • To assess the impact of different freezing rates on sperm viability and motility.
    • To compare the efficacy of the new technique with established cryopreservation methods.

    Summary:

    • A new method for freezing and storing human spermatozoa was investigated using various container types and controlled freezing rates.
    • Optimal freezing rates of 2.8°C/min and 12°C/min yielded the best results for samples with initial motility around 80%.
    • The technique achieved a 50% recovery of motility and fertilizability in normospermic samples and 34% in oligospermic samples after up to 25 days of storage.

    Impact:

    • This technique offers a potentially low-cost alternative for sperm cryopreservation.
    • The findings contribute to improving sperm banking and assisted reproduction outcomes.
    • Results are comparable to other recent advancements in sperm cryopreservation literature.

    Related Experiment Videos