An improved procedure for in situ RTPCR
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Updated: Nov 5, 2026

07:06
Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
Published on: February 6, 2026
L Volpi1, L Doneda, M G Grimoldi
1Dept. of Biology and Genetics, University of Milan, Milan, Italy.
Molecular and Cellular Probes
|February 1, 1996
Abstract:
Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.
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