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[P-glycoprotein expression in osteosarcoma]

M Pösl1, K Grahl, M Amling

  • 1Abteilung Osteopathologie, Pathologisches Institut, Universität, Hamburg.

Der Pathologe
|January 1, 1996
PubMed
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P-glycoprotein, a multidrug resistance mechanism, is expressed in most osteosarcomas, even before chemotherapy. However, its presence in osteosarcoma biopsies does not predict treatment response.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Multidrug resistance (MDR) in cancer is often mediated by P-glycoprotein, the product of the mdr1 gene.
  • Osteosarcoma is a primary bone cancer predominantly affecting children and adolescents.

Purpose of the Study:

  • To investigate P-glycoprotein expression in osteosarcomas using immunohistochemistry.
  • To determine if P-glycoprotein expression correlates with chemotherapy response in osteosarcoma patients.

Main Methods:

  • Immunohistochemistry was performed on 63 osteosarcoma biopsies from 54 patients using the JSB-1 monoclonal antibody.
  • P-glycoprotein expression was also assessed in normal bone growth plate tissues.

Main Results:

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  • P-glycoprotein expression was detected in 68.5% of osteosarcoma cases.
  • A higher incidence of P-glycoprotein positivity was observed in patients who received chemotherapy (80.9%) compared to those who did not (66.6%).
  • No significant correlation was found between P-glycoprotein expression levels and the response to chemotherapy in surgical specimens.

    • Conclusions:

    • P-glycoprotein is expressed in osteosarcomas prior to chemotherapy, similar to its presence in normal bone tissue.
    • Immunohistochemical determination of P-glycoprotein in osteosarcoma biopsies cannot reliably predict the response to chemotherapy.