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[A highly-productive attenuated variant of Japanese encephalitis virus]

P G Deriabin, N V Loginova

    Voprosy Virusologii
    |November 1, 1995
    PubMed

    Insights

    A new Japanese encephalitis virus (JEV) strain, clone 3, shows enhanced replication and cell-damaging abilities in chicken cells. This attenuated JEV strain maintains its novel biological properties through multiple passages, indicating potential for vaccine development.

    Area of Science:

    • Virology
    • Immunology

    Background:

    • Japanese encephalitis virus (JEV) is a significant cause of viral encephalitis globally.
    • Development of effective JEV vaccines and antiviral strategies remains crucial.

    Purpose of the Study:

    • To characterize a novel attenuated strain of Japanese encephalitis virus (JEV), clone 3, derived from chronic infection.
    • To evaluate the biological properties, including replication, cytopathogenicity, and antigenic characteristics, of this JEV clone.

    Main Methods:

    • Isolation of JEV clone 3 from chronically infected mouse brain cell cultures.
    • Assessment of viral replication and cytodestruction in chicken fibroblast and embryo cell cultures.
    • Quantification of viral titers and evaluation of antigenic activity and relationships.

    Main Results:

    • JEV clone 3 demonstrated active replication in chicken fibroblast cultures.
    • This attenuated JEV strain exhibited significant cytodestructive capacity against chicken embryo cells.
    • High viral titers (≥9.0 lg PFU/ml) and strong antigenic activity with broad antigenic relationships were observed.
    • Acquired biological properties were stable over multiple passages in mouse brain.

    Conclusions:

    • JEV clone 3 possesses unique biological properties, including enhanced replication and cytopathogenicity.
    • The stability of these properties suggests potential for further investigation in JEV research and vaccine development.

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