Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proteolysis of tropoelastin

R Mecham, J A Foster, C Franzblau

    Advances in Experimental Medicine and Biology
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    A novel enzyme linked to tropoelastin has been identified. This enzyme, with trypsin-like specificity, cleaves tropoelastin into distinct polypeptide fragments, offering insights into elastin degradation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genome-wide association studies of placebo and duloxetine response in major depressive disorder.

    The pharmacogenomics journal·2017
    Same author

    Gut Microbiome and Behavior: Focus on Neuroimmune Interactions.

    International review of neurobiology·2016
    Same author

    Lactobacillus rhamnosus strain JB-1 reverses restraint stress-induced gut dysmotility.

    Neurogastroenterology and motility·2016
    Same author

    Regional brain volumes changes in adult male FMR1-KO mouse on the FVB strain.

    Neuroscience·2016
    Same author

    3D visualization of the regional differences.

    Molecular psychiatry·2015
    Same author

    Clustering autism: using neuroanatomical differences in 26 mouse models to gain insight into the heterogeneity.

    Molecular psychiatry·2014

    Area of Science:

    • Biochemistry
    • Enzymology
    • Molecular Biology

    Background:

    • Tropoelastin is the precursor protein to elastin, a crucial component of connective tissues.
    • Understanding the enzymatic modifications of tropoelastin is key to comprehending elastin metabolism and related diseases.

    Purpose of the Study:

    • To characterize a newly discovered enzyme associated with tropoelastin.
    • To determine the enzymatic activity, specificity, and cleavage products of this tropoelastin-associated enzyme.

    Main Methods:

    • Enzyme assays were performed to determine pH optimum and substrate specificity.
    • Tropoelastin incubation with the enzyme followed by SDS-PAGE analysis to identify cleavage fragments.
    • Molecular weight determination of the parent molecule and resulting polypeptide fragments.

    Related Experiment Videos

    Main Results:

    • The enzyme exhibits optimal activity at a pH range of 7-9.
    • The enzyme demonstrates trypsin-like specificity, cleaving tropoelastin.
    • Incubation resulted in the fragmentation of tropoelastin (72,000 MW) into five distinct polypeptide bands (approx. 57,000, 45,000, 36,000, 25,000, and 13-14,000 MW).

    Conclusions:

    • A novel enzyme associated with tropoelastin has been identified and characterized.
    • This enzyme plays a role in the proteolytic processing of tropoelastin, generating specific high-molecular-weight fragments.
    • The findings contribute to the understanding of elastin biogenesis and degradation pathways.