Improvement of postischemic kidney function by reperfusion with a specifically developed solution (BT01)

Area of Science:

• Organ preservation and transplantation
• Nephrology
• Cardiovascular research

Background:

• Reperfusion injury is a significant challenge in organ preservation.
• Effective strategies for mitigating kidney damage during reperfusion are crucial for successful transplantation and recovery.

Purpose of the Study:

• To develop and evaluate a novel solution (BT01) for improving postischemic kidney reperfusion.
• To assess the efficacy of BT01 in reducing kidney damage and enhancing function after ischemia.

Main Methods:

• Micropigs underwent 60 minutes of unilateral kidney ischemia.
• The experimental group received initial reperfusion with BT01 solution mixed with blood (1:4 ratio), followed by systemic blood reperfusion.
• The control group received immediate reperfusion with pure blood.
• Glomerular filtration rate (GFR), kidney blood flow, and histological examinations were performed.

Main Results:

• Animals treated with BT01 (group 2) recovered diuresis, unlike 50% of control animals (group 1).
• Group 2 showed significantly higher GFR (0.51 vs. 0.16) and improved kidney blood flow (63.0 ml/min vs. 27.4 ml/min) compared to group 1.
• Histological analysis revealed reduced tubular necrosis and degenerative lesions in kidneys treated with BT01.

Conclusions:

• Flushing kidneys with BT01 solution mixed with blood effectively reduces postischemic reperfusion injury.
• This method enhances kidney function, blood flow, and histological integrity after ischemia.
• BT01 represents a promising therapeutic strategy for improving kidney preservation and outcomes.