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Sequence and neuronal expression of mouse endothelin-1 cDNA
1National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Ibaraki, Japan.
Biochimica Et Biophysica Acta
|July 17, 1996
Summary
Researchers isolated mouse endothelin-1 (ET-1) cDNA, revealing a conserved mature protein sequence. ET-1 mRNA was detected in key brain regions like the suprachiasmatic nucleus (SCN).
Area of Science:
- Molecular Biology
- Neuroscience
- Endocrinology
Background:
- Endothelin-1 (ET-1) is a potent vasoconstrictor peptide with diverse physiological roles.
- Understanding the genetic basis and brain expression of ET-1 is crucial for its neurobiological functions.
Purpose of the Study:
- To isolate and sequence the mouse endothelin-1 (ET-1) cDNA.
- To investigate the expression pattern of ET-1 mRNA in the rodent brain.
Main Methods:
- cDNA isolation and sequencing.
- In situ hybridization histochemistry.
Main Results:
- A cDNA encoding mouse prepro-endothelin-1 (202 amino acids) was successfully isolated and sequenced.
- The mature ET-1 sequence showed high identity (21 amino acids) with homologous peptides from other species.
- ET-1 mRNA expression was identified in multiple hypothalamic nuclei, notably the suprachiasmatic nucleus (SCN).
Conclusions:
- The study provides the genetic sequence for mouse ET-1, confirming its conservation across species.
- The findings demonstrate the presence of ET-1 mRNA in specific hypothalamic nuclei, suggesting a potential role in central nervous system regulation.