Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peptides

H von Döhren1

  • 1Institut für Biochemie und Molekulare Biologie Technische Universitt Berlin, Germany.

Biotechnology (Reading, Mass.)
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Peptides are key pharmaceuticals, but understanding biosynthesis and regulation will unify bioactive compound approaches. Future methods will leverage genetic knowledge and modular enzyme functions for complex natural product synthesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Relationship between activating and editing functions of the adenylation domain of apo-tyrocidin synthetase 1 (apo-TY1).

Biochimie·2005
Same author

Rapid screening and dereplication of bacterial isolates from marine sponges of the sula ridge by intact-cell-MALDI-TOF mass spectrometry (ICM-MS).

Applied microbiology and biotechnology·2004
Same author

Altered expression of two light-dependent genes in a microcystin-lacking mutant of Microcystis aeruginosa PCC 7806.

Microbiology (Reading, England)·2001
Same author

Determination of oligopeptide diversity within a natural population of Microcystis spp. (cyanobacteria) by typing single colonies by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Applied and environmental microbiology·2001
Same author

Dipeptide synthesis by an isolated adenylate-forming domain of non-ribosomal peptide synthetases (NRPS).

FEBS letters·2001
Same author

Synthesis of (di)adenosine polyphosphates by non-ribosomal peptide synthetases (NRPS).

Biochimica et biophysica acta·2001
See all related articles

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Peptides are the most commercially significant pharmaceuticals, with shared biosynthetic origins with beta-lactam antibiotics.
  • Advancements in understanding biosynthesis, genetics, and metabolite regulation are paving the way for a unified approach to bioactive compounds.

Purpose of the Study:

  • To explore the potential for a unified approach to bioactive compounds by understanding biosynthetic pathways and enzyme functions.
  • To highlight the emerging connections between genes in biosynthetic and degradative pathways.

Main Methods:

  • Analysis of genetic and enzymic backgrounds of biosynthesis.
  • Investigation of metabolite production regulation.
  • Examination of modular organization of catalytic functions and substrate transfer mechanisms.

Related Experiment Videos

Main Results:

  • Peptide synthetases share similarities with acylcoenzyme A synthetases, crucial for polyketide formation.
  • 4'-Phosphopantetheine is identified as a key transfer cofactor in multienzyme systems.
  • A conserved set of modification reactions (epimerization, methylation, hydroxylation, etc.) are found within or among biosynthetic proteins as ordered modules.

Conclusions:

  • Knowledge of biosynthetic genes, regulatory networks, and modular enzyme organization will enable novel synthetic strategies.
  • Exploiting living cells for synthesis requires mastery of cellular controls and compartments.
  • Biochemistry is approaching synthetic chemistry's capabilities, with potential for protein-based synthesis.