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Human sperm beta-glucuronidase is poorly extractable by Triton X-100
A Brandelli1, P V Miranda, J G Tezón
1Departamento de Bioquimica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
Cell Biology International
|May 1, 1996
Abstract:
A part of sperm glycosidase activities was detected as detergent-insoluble after sequential extractions with Triton X-100. Sixty per cent of total beta-glucuronidase activity was found in the detergent-insoluble fraction. This portion of beta-glucuronidase was resistant to extractions in the presence of 1 M KCl, chaotropic agents, colchicine or cytochalasine B, being only partially solubilized by 3 M KCl or DNAse I treatment. Results demonstrate that beta-glucuronidase is tightly associated to the Triton X-100 resistant fraction.