Immunochemical characterization of Ancylostoma caninum antigens

Adult worms of Ancylostoma caninum were dissected and manually separated into cephalic glands, cervical glands, intestine, esophagus and cuticula. These fractions as well as third stage larvae were fractionated with Triton X-114 into water soluble (hydrophilic), Triton soluble (hydrophobic) and unsoluble proteins. These fractions were characterized by immunoblotting with serum from rabbits immunized either with a pool of cervical, cephalic glands and intestine, or the esophagus fraction as well as with sera from percutaneously infected dogs and rabbits. Immunodominant antigens were found that reacted with dog or rabbit post infection sera and could be suited as antigens in serodiagnostic tests. Hidden antigens were found in the several fractions. Those from esophagus and intestine could be vaccine candidates that will be tested in immunization trials.