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Factors conditioning detection of Taenia saginata antigens in faeces

B Machnicka1, E Dziemian, C Zwierz

  • 1Institute of Parasitology of the Polish Academy of Sciences, Warsaw, Poland.

Applied Parasitology
|June 1, 1996
PubMed

The conditions which optimize detection of T. saginata antigens in faeces were subject of examination. The most appropriate appeared diluent of PBS + 0.3% Tween 20. The highest effectiveness was achieved using capturing and detecting antibodies to surface antigens in comparison with somatic and metabolic ones. Two step centrifugation and final dilution of samples 1:10 (w:v) were recommended. The stability of freezed faecal supernatants after the first centrifugation was limited to 2 months. The sensitivity of capture ELISA established in our examinations allow to signalize 1 ng/ml antigen in high absorbance values. The antigens recognized by the test had MW bigger than 100,000.

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