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Related Experiment Videos

Last male sperm precedence in a damselfly demonstrated by RAPD profiling

R E Hooper1, M T Siva-Jothy

  • 1Department Animal and Plant Sciences, University of Sheffield, UK.

Molecular Ecology
|June 1, 1996
PubMed
Summary

Last male sperm precedence (P2) in damselflies is high, with the most recent male fathering 98% of offspring. This occurs even when males do not fully remove rival sperm, influencing reproductive success.

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Area of Science:

  • Reproductive biology
  • Behavioral ecology
  • Population genetics

Background:

  • Sperm competition is a key factor influencing mating systems and reproductive success in many species.
  • Last male sperm precedence (P2) describes the likelihood that the most recent male to mate sires the offspring.

Purpose of the Study:

  • To determine the last male sperm precedence (P2) in the damselfly Calopteryx splendens xanthostoma.
  • To investigate the role of sperm competition in the reproductive strategy of this damselfly species.

Main Methods:

  • Random Amplified Polymorphic DNA (RAPD) technique was employed for DNA amplification.
  • DNA was amplified from mothers, putative fathers, and individual offspring embryos.
  • Band-matching coefficients were calculated to estimate last-male paternity.

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Main Results:

  • A high P2 value of 0.98 was observed in the oviposition bout immediately following copulation.
  • This high P2 was recorded despite males not completely removing previous males' sperm.

Conclusions:

  • Last male sperm precedence is a significant factor in the reproductive success of Calopteryx splendens xanthostoma.
  • The findings align with observations in other Calopterygidae species, highlighting conserved reproductive strategies.