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Time-dependent histamine release from stored human blood products
H J Nielsen1, L Edvardsen, K Vangsgaard
1Department of Surgical Gastroenterology, Hvidovre University Hospital, Copenhagen, Denmark.
The British Journal of Surgery
|February 1, 1996
Summary
Histamine release increases over time in stored whole blood and plasma-reduced whole blood, but not in SAGM blood. This spontaneous histamine release during blood storage may contribute to adverse transfusion reactions.
Area of Science:
- Transfusion Medicine
- Immunology
- Biochemistry
Background:
- Perioperative whole blood transfusion can worsen trauma-induced immunosuppression.
- Histamine2 receptor antagonists may mitigate these immunosuppressive effects.
- Understanding histamine levels in stored blood products is crucial.
Purpose of the Study:
- To analyze histamine content in different blood products during storage.
- To investigate spontaneous histamine release over 35 days.
- To correlate histamine levels with blood product type and storage duration.
Main Methods:
- Whole blood, plasma-reduced whole blood, and SAGM blood units were stored for 35 days at 4°C.
- Plasma and cell-bound histamine were measured at donation and periodically during storage.
- Enzyme-linked immunosorbent assay (ELISA) was used for histamine quantification.
Main Results:
- Median plasma histamine was 4.8 nmol/l at donation.
- Cell-bound histamine was present in whole blood and plasma-reduced whole blood, but undetectable in SAGM blood.
- Spontaneous histamine release significantly increased over 35 days in whole blood and plasma-reduced whole blood, but remained low in SAGM blood.
Conclusions:
- Human blood products containing leukocytes exhibit spontaneous histamine release during storage.
- This release is time-dependent and varies significantly between blood product types.
- Histamine release during blood storage may contribute to adverse transfusion outcomes.